Arabic Arabic English English French French German German
dark

18β-Glycyrrhetinic acid inhibits the apoptosis of cells infected with rotavirus SA11.

PMID: 

Pharm Biol. 2021 Dec ;59(1):1098-1105. PMID: 34411493

Abstract Title: 

18β-Glycyrrhetinic acid inhibits the apoptosis of cells infected with rotavirus SA11 via the Fas/FasL pathway.

Abstract: 

CONTEXT: 18β-Glycyrrhetinic acid (18β-GA), a pentacyclic triterpenoid saponin metabolite of glycyrrhizin, exhibits several biological activities.OBJECTIVE: We investigated the effects of 18β-GA on MA104 cells infected with rotavirus (RV) and its potential mechanism of action.MATERIALS AND METHODS: Cell Counting Kit-8 was used to assess tissue culture infective dose 50 (TCID) and 50% cellular cytotoxicity (CC) concentration. MA104 cells infected with RV SA11 were treated with 18β-GA (1, 2, 4, and 8 μg/mL, respectively). Cytopathic effects were observed. The virus inhibition rate, concentration for 50% of maximal effect (EC), and selection index (SI) were calculated. Cell cycle, cell apoptosis, and mRNA and protein expression related to the Fas/FasL pathway were detected.RESULTS: TCIDof RV SA11 was 10/100 µL; the CCof 18β-GA on MA104 cells was 86.92 µg/mL. 18β-GA showed significant antiviral activity; ECwas 3.14 μg/mL, and SI was 27.68. The ratio of MA104 cells infected with RV SA11 in the G0/G1 phase and the G2/M phase decreased and increased, respectively, after 18β-GA treatment. 18β-GA significantly induced apoptosis in the infected cells. Furthermore, after 18β-GA treatment, the mRNA and proteinexpression levels of Fas, FasL, caspase 3, and Bcl-2 decreased, whereas the expression levels of Bax increased.DISCUSSION AND CONCLUSIONS: The study demonstrates that 18β-GA may be a promising candidate for the treatment of RV SA11 infection and provides theoretical support for the clinical development of glycyrrhizic acid compounds for the treatment of RV infection.

read more

Read More

Total
0
Shares
Leave a Reply

Your email address will not be published. Required fields are marked *

Previous Post

Glycyrrhizin modulates chronic seizures-induced memory dysfunction.

Next Post

Anti-inflammatory and pro-apoptotic effects of 18beta-glycyrrhetinic acid.

Related Posts
Total
0
Share