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Basic Molecular Biology: PCR and Real-Time PCR – RT-PCR Fluorescent Probe-Based Detection

Detection of PCR products in real time can be accomplished by using fluorescent dyes or probes. Fluorescently labeled probes detect the amount of specific double-stranded DNA sequences. While fluorescent dyes detect only the amount of double-stranded DNA, in this video we are going to look at the principles behind fluorescent dye-based detection method.

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This video can also be viewed at
https://www.cdc.gov/labtraining/videos/basic-molecular-biology/molecular-fluorescent-detection/Molecular_Fluorescent_Detection_lowres.mp4

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