Arabic Arabic English English French French German German

Structural characterization and inhibition on α-glucosidase activity of acidic polysaccharide from Annona squamosa.


Carbohydr Polym. 2017 Oct 15 ;174:1-12. Epub 2017 Jun 15. PMID: 28821021

Abstract Title: 

Structural characterization and inhibition onα-glucosidase activity of acidic polysaccharide from Annona squamosa.


The crude polysaccharide (TASP3) was extracted from the fruit pulp of Annona squamosa and then isolated and purified by the combination of grading-alcoholic precipitation and Sephadex G-200. The structure of purified polysaccharide (GASP3-3-I) was determined based on the physicochemical and instrumental analyses. The results indicated that GASP3-3-I was an acidic heteropolysaccharide and its average molecular weight was 2.28×10Da. The monosaccharide composition was analysed by GC-MS and ion chromatography, respectively. It was revealed that GASP3-3-I was consisted of rhamnose, arabinose, xylose, mannose, glucose, galactose, glucuronic acid and galacturonic acid with a molar ratio of 5.06:45.5:5.26:0.63:6.09:31.76:0.49:5.19. Moreover, periodate oxidation reaction, Smith degrading reaction, methylation, FT-IR and NMR were used to conduct the structural characterization of GASP3-3-I. The results indicated that glycosyl residues of GASP3-3-I were mainly composed of (1→) l-arabinose, (1→6), (1→3) and (1,3→6) d-galactose, (1→) d-xylose, (3→) and (3→6) d-glucose, (1→2) l-rhamnose. The α-glucosidase inhibitory activity assay showed that GASP3-3-I had a certain inhibition on α-glucosidase activity.

read more

Read More

Leave a Reply

Your email address will not be published. Required fields are marked *

Previous Post

Boysenberry and apple juice concentrate reduced acute lung inflammation.

Next Post

India virus surge drives record global daily cases

Related Posts